New DNA test results tied to a second vehicle of interest in the Megan McDonald murder case were revealed in court Wednesday — shedding light on a key piece of evidence that jurors questioned in the first trial.

Prosecutors say possible human tissue found in a Dodge Neon during the original 2003 investigation was recently tested and does not belong to the 20-year-old victim. The vehicle had been linked early on to another potential suspect.

Megan McDonald case: Murder trial begins in Orange County 22 years after brutal killing

State police didn’t send the sample out for testing until April 21 — five days after Edward Holley’s first trial ended in a mistrial.

The defense says the delay raises concerns and notes the results also show the sample was highly degraded after 22 years. Attorneys are asking for more time to allow their expert to conduct a full review.

The test results could help clear up key questions that came up during the first trial and are expected to play a role in shaping the case against Holley, who is accused of beating McDonald to death and dumping her body on a rural road in Wallkill.

Holley was arrested in 2023 following a renewed cold case investigation and remains in custody. His first trial ended without a verdict after jurors could not reach a unanimous decision.

A new trial date has now been scheduled for Jan. 12.